Search
Close this search box.

Directed evolution of a type A feruloyl esterase for increased thermostability and organic solvent tolerance

Julien Durand, Victor Chaumeton, Olga Gherbovet, Mélanie Ragon, Peter Jütten, Joana L.A. Bras, Catarina I.P.D. Guerreiro, Carlos M.G.A. Fontes, Alexander Piechot, Régis Fauré, Sophie Bozonnet, Michael J. O’Donohue

Taros

Abstract:

Feruloyl esterases (FAE) are carboxylic esterases (EC 3.1.1) that cleave the bond between ferulic acid and L-arabinofuranosyl residues of arabinoxylans. These enzymes, in addition to helping the  econstruction of lignocellulosic biomass, have a natural capacity to synthesize a wide range of bioactive molecules with interesting properties (notably anti-oxidant) in the fields of food, pharmaceuticals or cosmetics.
Within the OPTIBIOCAT European project, a methodology to express, generate and screen diversity for Aspergillus niger feruloyl esterase A was developed, tested and implemented in a high-throughput fashion, using ad hoc chromogenic probes [1]. A total of ~10,000 mutant clones were generated and screened to isolate 13 mutants with improved thermal and/or solvent stability. The most interesting mutations were individually studied while their random recombination was performed in a second round of evolution through Staggered Extension Process (StEP) [2].

Want to know more about this publication? Contact our scientists

Medicinal chemistry

Drug Discovery

Taros’ international and multidisciplinary team has more than 180 years of pharmaceutical R&D experience. Collectively as a team, we worked on more than 120 biomolecular…

custom chemical services

Custom Synthesis

We support our customers by providing first kg quantities of fine and specialty chemicals, cost reduction of existing syntheses, streamlining chemical processes considering…

Looks interesting?

As a leading CRO in Europe, Taros provides comprehensive drug discovery chemistry services for large Pharma and Biotech corporations as well as for Research Institutes and Academia

Our experienced chemists practice a pragmatic culture and customer-centric approach in order to exceed project expectations and, despite challenges, bridge very narrow timelines. They are trained to consider economic, safety and ecological boundaries and conditions laid out for all stages of the project.
Scroll to Top