High-throughput preparative HPLC-MS purification workflow of libraries within the European Lead Factory

Raquel Ortega, Martyna Bielska, Xenia Iwanowa, Anna Karawajczyk, Andreas Tei

Molecules 2018, 23, 2545  doi:10.3390/molecules23102545;

The Agilent OpenLAB CDS Automated Purification Software developed by Agilent Technologies in a close collaborative project with Taros was fully implemented creating automated purification workflows based on reversed phase high performance liquid chromatography (HPLC) and mass spectrometry (MS). The system is suitable for a smart combination of UV- and mass-based fraction triggering and the automated transfer of data between each single process streamlining the workflow for highest productivity as the purification of crude products is usually the most time consuming part of organic compound preparation. A fine tuning has been made from the chemist, modifying the slope offsets due to the chromatographic diversity of the different compound classes. The slope offsets have been saved in template files. Different template files have been used to purify difficult batches of compounds. The results and statistics collected from analytic and purification (retention time, reactivity, validation of chemistry and purification) have a high intrinsic value for the design, validation synthesis and production of new libraries.

Want to know more about this publication? Contact our scientists

Medicinal chemistry

Drug Discovery

Taros’ international and multidisciplinary team has more than 180 years of pharmaceutical R&D experience. Collectively as a team, we worked on more than 120 biomolecular…

custom chemical services

Custom Synthesis

We support our customers by providing first kg quantities of fine and specialty chemicals, cost reduction of existing syntheses, streamlining chemical processes considering…

Looks interesting?

As a leading CRO in Europe, Taros provides comprehensive drug discovery chemistry services for large Pharma and Biotech corporations as well as for Research Institutes and Academia

Our experienced chemists practice a pragmatic culture and customer-centric approach in order to exceed project expectations and, despite challenges, bridge very narrow timelines. They are trained to consider economic, safety and ecological boundaries and conditions laid out for all stages of the project.
Scroll to Top